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ANALYSIS SNAKE VENOM
BOTHROPS JARARACA
Sample: Total Snake
Specie: Bothrops jararaca
Serpentary: UREAUS
Register: IBAMA (Brazilian Institute Environment)
Origin: Province of Espírito Santo – Brazil
CONCENTRATION OF PROTEIN:
Total Proteins:……….....… 81.0%
Fractions |
Identification |
% |
I |
Trombina |
12.1% |
II |
Jararafibrase |
21.7% |
III |
Phospholipase |
43.6% |
IV |
Metaloprotease |
2.5% |
V |
Lectina |
2.7% |
VI |
Lectina |
3.3% |
VII |
Lectina |
14.0% |
Quantity of sample applied: 50mg
Dissolved: 400ml NH4H(CO3) 2 0,2M
Fractionate Conditions:
Gel Chromatographic Filtration:
Superdex G75 (Waters)
Drain plug of balance:
NH4H(CO3)2 0.2M
Drain plug of Eluition:
NH3H(CO3)2 0.2M
Dosage Method Total Protein:
Bradford
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